Blar i NTNU Open på forfatter "Sousa, Mirta"

Viser treff 1-19 av 19

    • AID expression in B-cell lymphomas causes accumulation of genomic uracil and a distinct AID mutational signature 

      Pettersen, Henrik Sahlin; Galashevskaya, Anastasia; Doseth, Berit; Sousa, Mirta; Sarno, Antonio; Visnes, Torkild; Aas, Per Arne; Liabakk, Nina-Beate; Slupphaug, Geir; Sætrom, Pål; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2014)
      tThe most common mutations in cancer are C to T transitions, but their origin has remained elusive.Recently, mutational signatures of APOBEC-family cytosine deaminases were identified in many com-mon cancers, suggesting ...

    • An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells 

      Sousa, Mirta; Zub, Kamila Anna; Aas, Per Arne; Hanssen-Bauer, Audun; Demirovic, Aida; Sarno, Antonio; Tian, Erming; Liabakk, Nina-Beate; Slupphaug, Geir (Journal article; Peer reviewed, 2013)
      Alterations in checkpoint and DNA repair pathways may provide adaptive mechanisms contributing to acquired drug resistance. Here, we investigated the levels of proteins mediating DNA damage signaling and -repair in RPMI8226 ...

    • Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis 

      Sousa, Mirta; Steen, Kristian Wollen; Hagen, Lars; Slupphaug, Geir (Journal article; Peer reviewed, 2011)
      Background Immunoprecipitation and subsequent 2D-PAGE/mass spectrometry are powerful tools to study post-translational protein modifications. Often disregarded in this workflow is the impact of the chemical cross-linker ...

    • Atrial Myocyte Function and Ca2+ Handling Is Associated with Inborn Aerobic Capacity 

      Johnsen, Anne Berit; Rolim, Natale Pinheiro Lage; Stølen, Tomas; Alves, Marcia; Sousa, Mirta; Slupphaug, Geir; Britton, Steven L; Koch, Lauren G; Smith, Godfrey L; Wisløff, Ulrik; Høydal, Morten Andre (Peer reviewed; Journal article, 2013-10-16)
      Background: Although high aerobic capacity is associated with effective cardiac function, the effect of aerobic capacity on atrial function, especially in terms of cellular mechanisms, is not known. We aimed to investigate ...

    • DNA Repair Mechanisms are Activated in Circulating Lymphocytes of Hospitalized Covid-19 Patients 

      Olsen, Maria Belland; Huse, Camilla; Sousa, Mirta; Murphy, Sarah Louise Mikalsen; Sarno, Antonio; Obermann, Tobias Sebastian; Yang, Kuan; Holter, Jan Cato; Jørgensen, Marthe Jøntvedt; Christensen, Erik Egeland; Wang, Wei; Ji, Ping; Heggelund, Lars; Hoel, Hedda; Dyrhol-Riise, Anne Ma; Gregersen, Ida; Aukrust, Pål; Bjørås, Magnar; Halvorsen, Bente; Dahl, Tuva Børresdatter (Peer reviewed; Journal article, 2022)
      Purpose: Reactive oxygen species (ROS) are an important part of the inflammatory response during infection but can also promote DNA damage. Due to the sustained inflammation in severe Covid-19, we hypothesized that ...

    • Duodenal inflammation in common variable immunodeficiency has altered transcriptional response to viruses 

      Kaarbø, Mari; Yang, Mingyi; Hov, Johannes Espolin Roksund; Holm, Kristian; Sousa, Mirta; Macpherson, Magnhild Eide; Reims, Henrik Mikael; Kran, Anne-Marte Bakken; Halvorsen, Bente; Karlsen, Tom Hemming; Aukrust, Pål; Lundin, Knut; Fevang, Børre; Bjørås, Magnar; Jørgensen, Silje Fjellgård (Peer reviewed; Journal article, 2022)
      Background A substantial proportion of common variable immunodeficiency (CVID) patients has duodenal inflammation of largely unknown etiology. However, because of its histologic similarities with celiac disease, gluten ...

    • A fast, low-cost, robust and high-throughput method for viral nucleic acid isolation based on NAxtra magnetic nanoparticles 

      Ravlo, Erlend; Sousa, Mirta; Andresen, Lise Lima; Holberg-Petersen, Mona; Klundby, Ingvild; Aas, Per Arne; Hagen, Lars; Erlandsen, Sten Even; Fossum, Janne; Ali, Zeeshan; Sharma, Anuvansh; Ottesen, Vegar; Bandyopadhyay, Sulalit; Bjørås, Magnar (Peer reviewed; Journal article, 2023)
      The year of 2020 was profoundly marked by a global pandemic caused by a strain of coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19). ...

    • HDACi Mediate UNG2 Depletion, Dysregulated Genomic Uracil and Altered Expression of Oncoproteins and Tumor Suppressors in B- And T-cell Lines 

      Iveland, Tobias Solli; Hagen, Lars; Sharma, Animesh; Sousa, Mirta; Sarno, Antonio; Kristian Lied, Wollen; Liabakk, Nina-Beate; Slupphaug, Geir (Peer reviewed; Journal article, 2020)
      Background HDAC inhibitors (HDACi) belong to a new group of chemotherapeutics that are increasingly used in the treatment of lymphocyte-derived malignancies, but their mechanisms of action remain poorly understood. Here ...

    • A loss-of-function mutation in human Oxidation Resistance 1 disrupts the spatial–temporal regulation of histone arginine methylation in neurodevelopment 

      Lin, Xiaolin; Wang, Wei; Yang, Mingyi; Damseh, Nadirah; Sousa, Mirta; Jacob, Fadi; lång, anna ulrika; Kristiansen, Elise; Pannone, Marco; Kissova, Miroslava; Almaas, Runar; Kusnierczyk, Anna; Siller, Richard; Shahrour, Maher; Al-Ashhab, Motee; Abu-Libdeh, Bassam; Tang, Wannan; Slupphaug, Geir; Elpeleg, Orly; Bøe, Stig Ove; Eide, Lars; Sullivan, Gareth John; Rinholm, Johanne Egge; Song, Hongjun; Ming, Guo-li; van Loon, Barbara; Edvardson, Simon; Ye, Jing; Bjørås, Magnar (Peer reviewed; Journal article, 2023)
      Background Oxidation Resistance 1 (OXR1) gene is a highly conserved gene of the TLDc domain-containing family. OXR1 is involved in fundamental biological and cellular processes, including DNA damage response, antioxidant ...

    • Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. 

      Zub, Kamila Anna; Sousa, Mirta; Sarno, Antonio; Sharma, Animesh; Demirovic, Aida; Rao, Shalini; Young, Clifford; Aas, Per Arne; Ericsson, Ida; Sundan, Anders; Jensen, Ole Nørregaard; Slupphaug, Geir (Journal article; Peer reviewed, 2015)
      Alkylating agents are widely used chemotherapeutics in the treatment of many cancers, including leukemia, lymphoma, multiple myeloma, sarcoma, lung, breast and ovarian cancer. Melphalan is the most commonly used chemotherapeutic ...

    • Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication. 

      Bjørås, Karine; Sousa, Mirta; Sharma, Animesh; Fonseca, Davi de Miranda; Søgaard, Caroline Danielsen; Bjørås, Magnar; Otterlei, Marit (Journal article; Peer reviewed, 2017)
      Base lesions in DNA can stall the replication machinery or induce mutations if bypassed. Consequently, lesions must be repaired before replication or in a post-replicative process to maintain genomic stability. Base excision ...

    • N6-methyladenosine in RNA of atherosclerotic plaques: An epitranscriptomic signature of human carotid atherosclerosis 

      Quiles Jimenez, Ana Maria Teresa; Gregersen, Ida; Sousa, Mirta; Abbas, Azhar; Kong, Xiang Yi; Alseth, Ingrun; Holm, Sverre; Dahl, Tuva Børresdatter; Skagen, Karolina Ryeng; Skjelland, Mona; Aukrust, Pål; Bjørås, Magnar; Halvorsen, Bente (Peer reviewed; Journal article, 2020)
      Background More than 170 post-transcriptional RNA modifications regulate the localization, processing and function of cellular RNAs, and aberrant RNA modifications have been linked to a range of human diseases. The RNA ...

    • NAxtra magnetic nanoparticles for low-cost, efficient isolation of mammalian DNA and RNA 

      Starheim, Eirin Johannessen; Ravlo, Erlend; Schjølberg, Jørn-Ove; Solvang, Vanessa; Wang, Wei; Scrimgeour, Nathan Robert; Manaf, Adeel; Erlandsen, Sten Even; Aas, Per Arne; Hagen, Lars; Sousa, Mirta; Bjørås, Magnar (Peer reviewed; Journal article, 2023)
      A cost-effective, viral nucleic acid (NA) isolation kit based on NAxtra magnetic nanoparticles was developed at the Norwegian University of Science and Technology in response to the shortage of commercial kits for isolation ...

    • Novel UCHL1 mutations reveal new insights into ubiquitin processing 

      Rydning, Siri Lynne; Backe, Paul Hoff; Sousa, Mirta; Iqbal, Zafar; Øye, Ane-Marte; Sheng, Ying; Yang, Mingyi; Lin, Xiaolin; Slupphaug, Geir; Nordenmark, Tonje Haug; Vigeland, Magnus Dehli; Bjørås, Magnar; Tallaksen, Chantal; Selmer, Kaja Kristine (Journal article; Peer reviewed, 2017)
      Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 has been implicated in early-onset progressive neurodegeneration (MIM no. 615491), so far only in one family. In this study a second family is characterized, ...

    • OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation 

      Yang, Mingyi; Lin, Xiaolin; Segers, Filip; Suganthan, Rajikala; Hildrestrand, Gunn Annette; Rinholm, Johanne Egge; Aas, Per Arne; Sousa, Mirta; Holm, Sverre; Bolstad, Nils; Warren, David; Berge, Rolf Kristian; Johansen, Rune Forstrøm; Yndestad, Arne; Kristiansen, Elise; Klungland, Arne; Gomez, Luisa Fernanda Luna; Eide, Lars; Halvorsen, Bente; Aukrust, Pål; Bjørås, Magnar (Peer reviewed; Journal article, 2020)
      Oxidation resistance gene 1 (OXR1) protects cells against oxidative stress. We find that male mice with brain-specific isoform A knockout (Oxr1A−/−) develop fatty liver. RNA sequencing of male Oxr1A−/− liver indicates ...

    • P38 MAPK signaling and phosphorylations in the BRCT1 domain regulate XRCC1 recruitment to sites of DNA damage 

      Sousa, Mirta; Bjørås, Karine; Hanssen-Bauer, Audun; Solvang-Garten, Karin; Otterlei, Marit (Journal article; Peer reviewed, 2017)
      XRCC1 is a scaffold protein involved in base excision repair and single strand break repair. It is a phosphoprotein that contains more than 45 phosphorylation sites, however only a few of these have been characterized and ...

    • Proteome alterations associated with transformation of multiple myeloma to secondary plasma cell leukemia 

      Zatula, Alexey; Dikic, Aida; Mulder, Teska Celine; Sharma, Animesh; Vågbø, Cathrine Broberg; Sousa, Mirta; Waage, Anders; Slupphaug, Geir (Peer reviewed; Journal article, 2017)
      Plasma cell leukemia is a rare and aggressive plasma cell neoplasm that may either originate de novo (primary PCL) or by leukemic transformation of multiple myeloma (MM) to secondary PCL (sPCL). The prognosis of sPCL is ...

    • The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival 

      Vange, Pål; Bruland, Torunn; Doseth, Berit; Fossmark, Reidar; Sousa, Mirta; Beisvag, Vidar; Sørdal, Øystein Finset; Qvigstad, Gunnar; Waldum, Helge; Sandvik, Arne Kristian; Bakke, Ingunn (Journal article; Peer reviewed, 2017)
      The cytoprotective protein clusterin is often dysregulated during tumorigenesis, and in the stomach, upregulation of clusterin marks emergence of the oxyntic atrophy (loss of acid-producing parietal cells)-associated ...

    • Uracil-DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil 

      Sarno, Antonio; Lundbæk, Marie Benner; Liabakk, Nina-Beate; Aas, Per Arne; Mjelle, Robin; Hagen, Lars; Sousa, Mirta; Krokan, Hans Einar; Kavli, Bodil Merete (Journal article; Peer reviewed, 2019)
      UNG is the major uracil-DNA glycosylase in mammalian cells and is involved in both error-free base excision repair of genomic uracil and mutagenic uracil-processing at the antibody genes. However, the regulation of UNG in ...